Unprotected streams within the agricultural Midwest region of the United States are subject to sedimentation, nutrification, and agricultural chemicals. Grass riparian filter strips (GRFSs) have been implemented as a best management practice to minimize sedimentation and associated materials that are harmful to aquatic ecosystems; however, few studies have examined the benthic community response to GRFS installation. This study introduces a least-desired index (LDI) multimetric approach of evaluating benthic communities in response to GRFS installation. LDI was determined in a reciprocal fashion to that of a benthic macroinvertebrate index of biotic integrity (B-IBI). When reference conditions are not available for the use of B-IBI, anti-reference sites, representing least-desired conditions, can be used in constructing an LDI. A B-IBI and LDI were constructed in the Claypan Till Plains Subsection of Missouri and comparatively used to evaluate two test sites where tall fescue GRFS were installed. Five metrics were used to develop the B-IBI and six for the LDI. The LDI tended to be more conservative at evaluation in comparison to the B-IBI. Paired t-tests showed that LDI and B-IBI were significantly different at scoring test sites. The LDI assessed both test sites as showing no response to GRFS installation, whereas the B-IBI suggested moderate improvement. The LDI was considered to be a better index for evaluation because the streams used to develop the B-IBI were not suitable reference sites. An argument for the use of chironomid based metrics in low gradient agricultural streams is presented.